Molecular cloning of a Coxiella burnetii gene encoding a macrophage infectivity potentiator (Mip) analogue

Article Abstract:

The open reading frame of the Coxiella burnetii gene encodes a protein which is homologous to the macrophage infectivity potentiator (Mip) encoded by Legionella pneumophila (LpMip) and Chlamydia trachomatis Mip. The C. burnetii CbMip protein reacts with antibodies against LpMip. CbMip is probably a virulence factor of the pathogenic bacteria. The C-terminal of the protein is similar to the FK506-binding proteins present in prokaryotes and eukaryotes. CbMip overexpressed in Escherichia coil demonstrates peptidyl-prolyl cis-trans isomerase activity.

Usage, Bacteria, Pathogenic, Pathogenic bacteria, Cloning, Bacterial proteins

---

Secretion of proteins by Coxiella burnetii

Article Abstract:

Incubation of viable Coxiella burnetii organisms in host-cell-free medium at low pH leads to translocation of proteins secreted by the bacteria to the exterior of the cell. Three polypeptides with molecular masses of 34, 24 and 12 kDa are the major constituents of the exported protein. A membrane fraction has the de novo-synthesized proteins. The physiological process of protein transport is inhibited by dicylohexylcarbodiimide and carbonyl cyanide m-chlorophenylhydrazone.

Physiological aspects, Prokaryotes, Secretion, Physiological regulation, Biological transport

---

Involvement of outer-membrane proteins in the aggregation of Azospirillum brasilense

Article Abstract:

A study was conducted to analyze the biological factors involved in the aggregation of Azospirillum brasilense strain CD. The cells were grown under aggregation-inducing and non-aggregation-inducing environments. They were then washed by centrifugation and resuspended in a potassium phosphate buffer solution. Results indicated that outer-membrane proteins are involved in the aggregation process of cells of A brasilense.

Cells (Biology), Cells

Similar abstracts:

• Abstracts: Molecular fingerprinting of Cryptosporidium oocysts isolated during water monitoring. Physical inactivation of Toxoplasma gondii oocysts in water
• Abstracts: The tumor-suppressor function of E-cadherin. A mutation in CCDC50, a gene encoding an effector of epidermal growth factor-mediated cell signaling, causes progressive hearing loss
• Abstracts: Development of PCR primers from internal transcribed spacer region 2 for detection of Phytophthora species infecting potatoes
• Abstracts: Molecular characterization of adiY, a regulatory gene which affects expression of the biodegradative acid-induced arginine decarboxylase gene (adiA) of Escherichia coli
• Abstracts: Early determination of nasal-temporal retinotopic specificity in the eye anlage of the chick embryo. Interactive cellular modulation of chondrogenic differentiation in vitro by subpopulations of chick embryonic calvarial cells

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.