Protein splicing of inteins and hedgehog autoproteolysis: structure, function, and evolution
Article Abstract:
A research was conducted to study the protein splicing of inteins and hedgehog autoproteolysis using several approaches that defined the splicing mechanism and the three-dimensional structure of the Saccharomyces cerevisiae VMA intein. The structure of the Drosophila hedgehog protein autoprocessing domain was compared with the splicing domain. Results showed that large inteins were characterized by homing endonucleases that represent a core endonuclease domain.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Bacteriophage-based genetic system for selection of nonsplicing inteins
Article Abstract:
A genetic selection system that detects splicing and nonsplicing activities of inteins is constructed based on the ability to rescue a T4 phage strain with a conditionally inactive DNA polymerase. The conclusion suggest that development of genetic systems with the potential of identifying protein splicing inhibitors is a first step towards controlling proliferation of pathogenic microbes harboring inteins in essential proteins.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Experimental and theoretical bases of specific affinity, a cytoarchitecture-based formulation of nutrient collection proposed to supercede the Michaelis-Menten paradigm of microbial kinetics
Article Abstract:
A relationship between the concentration-limited rate of nutrient transport and organism cytoarchitecture based on available theory, in situ and laboratory kinetic data and derived concepts are described. The results obtained are in contrast to the Michaelis-Menten theory and they demonstrated that most oligobacteria in the environment use multiple substrates simultaneously to attain sufficient energy and material for growth.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibition. Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates
- Abstracts: Notch signaling in hematopoiesis and lymphopoiesis: Lessons from Drosophila. Meetings: Animal models of T-cell-mediated skin diseases
- Abstracts: Spatially resolved characterization of biogenic manganese oxide production within a bacterial biofilm. Influence of an oyster reef on development of the microbial heterotrophic community of an estuarine biofilm.
- Abstracts: ScCypB is a novel second cytosolic cyclophilin from Streptomyces chrysomallus which is phylogenetically distant from ScCypA