Quantitative interaction effects of carbon dioxide, sodium chloride, and sodium nitrite on neurotoxin gene expression in nonproteolytic clostridium botulinum type B
Article Abstract:
The effects of carbon dioxide, sodium chloride, and sodium nitrite on type B botulinum neurotoxin gene expression in nonproteolytic clostridium botulinum are investigated in a tryptone-peptone-yeast extract medium. Various concentrations of these food preservations are studied by using a complete factorial design in order to quantitatively study interaction effects, as well as main effects, on a number of responses.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Relative neurotoxin gene expression in clostridium botulinum type B, determined using quantitative reverse transcription-PCR
Article Abstract:
A quantitative reverse transcription-PCR method is developed to monitor the relative expression of the type B botulinum neurotoxin (BoNT/B) gene in clostridium botulinum. The levels of sntB mRNA in five type B strains are accurately monitored by using primers specific for cntB and for the reference gene encoding the 16S rRNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Development of a combined selection and enrichment PCR procedure for Clostridium botulinum types B, E, and F and its use to determine prevalence in fecal samples from slaughtered pigs
Article Abstract:
Researchers used a PCR procedure to show that many pigs are colonized with Clostridium botulinum type B but not types E or F. Seventy-one percent of the fecal samples that tested positive for C. botulinum had a spore load of less than four spores per gram.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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