SP75 is encoded by the DP87 gene and belongs to a family of modular Dictyostelium discoideum outer layer spore coat proteins
Article Abstract:
Partial sequencing of the major coat protein SP75 of Dictyostelium discoideum showed that it is encoded by the DP87 gene. Five of the abundant outer layer coat proteins revealed that they are homologous and comprised of alternating sequence motifs related to epithelial mucin repeats, basic proline repeats in salivary acidic proline-rich proteins, the NH2-terminal subdomain of epidermal growth factor modules and other cysteine repeats. A cell surface microenvironment is predicted to be created by these outer layer spore coat proteins in support of cellulose morphogenesis.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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The small GTP-binding protein Rho is expressed differentially during spore germination of Phycomyces blakesleeanus
Article Abstract:
A study was conducted to analyze the presence of the 22 kDA GTP-binding RHo protein in dormant spores of Phycomyces blakesleeanus. A soluble and membrane-associated 22 kDa protein was determined by immunoblotting with a polyclonal antibody against RhoA. The 22 kDa protein was then expressed at all stages of growth. Results indicated that RhoA was observed as patches in the cell membrane when the spore started to swell.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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The patB gene of Dictyostelium discoideum encodes a P-type H+-ATPase isoform essential for growth and development under acidic conditions
Article Abstract:
A study was conducted on the cloning and characterization of the putative P-type H+-ATPase gene, patB, of the amoebae, Dictyostelium discoideum. The findings indicate that patB encodes an acid-inducible P-type H+-ATPase which is essential for the survival of Dictyostelium cells in moderately acidic external environments. However, patB seems to be a nonessential gene in the controlled conditions of the laboratory.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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