Targeted mutagenesis of the endogenous mouse Mis gene promoter: in vivo definition of genetic pathways of vertebrate sexual development
Article Abstract:
In vivo definition of genetic pathways of vertebrate sexual development is discussed relative to targeted mutagenesis of the endogenous mouse Mis gene. promoter. The goal of this study was to discover the in vivo function of the conserved HMG- and SR1- binding sites in the Mis promoter. Complexity in the regulation of Mis not seen in in vitro and transgenic experiments with mice was seen. The SF1-binding site in the Mis promoter is not required for Mullerian duct regression, but is required for upregulation of Mis transcription. SF1 is a quantitative regulator of Mis transcription.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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Nuclear trafficking of cubitus interruptus in the transcriptional regulation of hedgehog target gene expression
Article Abstract:
A study was conducted to determine the role of Cubitus interruptus, a 155 kDa cytoplasmic zinc finger protein (Ci155), in mediating transcriptional activation of hedgehog (Hh) target genes. Hh signaling was found to reduce phosphorylation of Ci155 and increase the rate of Ci155 nuclear import. Findings also suggested that active nuclear export is required for maintenance of the inactive state of the pathway and that Hh-induced activation of targets is mediated through an increased nuclear import of Ci155.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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Hedgehog-regulated processing of Gli3 produces an anterior/posterior repressor gradient in the developing vertebrate limb
Article Abstract:
Processing of Gli3 regulated by Hedgehog gives rise to an anterior/posterior repressor gradient in developing limbs in vertebrates. Focus in a recent study was mostly on Gli2 and Gli3, both implicated in transduction of the Sonic hedgehog (Shh) signal, and the study investigated how Gli protein processing response to Shh signaling might serve as a pattern specification means. Spatial regulation of Gli protein processing in the limb of a developing vertebrate was studied.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2000
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