Simplified hot start PCR
Article Abstract:
Polymerase chain reaction (PCR) with thermally activated DNA polymerase gives greater product yield, and is more sensitive and specific. AmpliTaq Gold DNA polymerase used for PCR does not show any enzyme activity at room temperature but can be substituted in most PCR systems for thermostable DNA polymerase. A pre-PCR heat cycle restores the polymerase activity, and is programmable such that the conversion from enzyme activation to PCR cycling activity is above the optimal primer annealing temperature.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Historical highlights in therapies
Article Abstract:
Biologics have broadened the selection of drugs that might fight help in fighting autoimmunity. The new approaches for treating autoimmune diseases contain two basic elements, such as advanced therapies that attack specific components in the immune systems and developing various therapies with different mechanisms of action for same disease that benefit more from one form of therapy than another.
Publication Name: The Scientist
Subject: Biological sciences
ISSN: 0890-3670
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
The new men's movement
Article Abstract:
The Promise Keepers fundamentalist church founded by former football coach Bill McCartney has become the dominant religion in the US in the 1990s. However, feminists are concerned that the movement has aims to curtail the gains made in women's rights.
Publication Name: New Woman
Subject: General interest
ISSN: 0028-6974
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: The new woman. That woman. The community speaks: one woman's complaint leads to a new park
- Abstracts: I oughta be in pictures. Home improvement. Ingenue engineering
- Abstracts: The Queen carries on. Artless philanthropy
- Abstracts: The end of Camelot. Home Depot Inc. - HD
- Abstracts: The zit list. Shaker Screen